Organophosphate poisoning is a major public health problem in developing countries. Organophosphates cause prolonged seizures and leads to neurodegeneration and functional defects. Current therapies are largely ineffective. Several advanced neuroimaging techniques demonstrate promise to detect subtle changes in brain activity and morphology related to organophosphate nerve agent poisoning, which would allow for the in vivo assessment of new therapeutics. In this study we use in vivo MRI and immunohistochemistry to demonstrate that fluorinated volatile anesthetics are an effective post-exposure neuroprotectant and can be used for organophosphates poisoning treatment.