Olfactory perception in mice involves the activation of brain regions. We previously developed a method that uses repetitive odor stimulation and independent component analysis (ICA) for BOLD-fMRI studies. Using BOLD-ICA, we sought to detect odor-evoked responses in the mouse whole brain. Previously, the administration of an odorant substance had been performed by manually operating a syringe pump to infuse the saturated vapor of an odorant substance. We constructed a system that automatically controls the syringe pump, to ensure the accuracy of stimulation timing and duration. We demonstrate the improved detectability in the BOLD-ICA method by the automated system.
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