Meeting Banner
Abstract #3685

Odor stimulation by automated syringe pumps in combination with the independent component analysis for BOLD-fMRI study of mouse whole brain

Fuyu Hayashi1, Mitsuhiro Takeda1, Naoya Yuzuriha1, Sosuke Yoshinaga1, and Hiroaki Terasawa1

1Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan

Olfactory perception in mice involves the activation of brain regions. We previously developed a method that uses repetitive odor stimulation and independent component analysis (ICA) for BOLD-fMRI studies. Using BOLD-ICA, we sought to detect odor-evoked responses in the mouse whole brain. Previously, the administration of an odorant substance had been performed by manually operating a syringe pump to infuse the saturated vapor of an odorant substance. We constructed a system that automatically controls the syringe pump, to ensure the accuracy of stimulation timing and duration. We demonstrate the improved detectability in the BOLD-ICA method by the automated system.

This abstract and the presentation materials are available to members only; a login is required.

Join Here