Abstract #3986

GlucoCEST MRI detects metabolic degradation in the mouse brain after rapid microwave fixation

Chao-Hsiung Hsu¹, Stephen Lin¹, Tirone Johnson², Ke-Yi Wu^1,3, Liang-Ron Chen^1,4, Paul Wang^1,3, Joseph Scafidi², and Tsang-Wei Tu¹

¹Howard University, Washington, DC, United States, ²Children's National Medical Center, Washington, DC, United States, ³Fu Jen Catholic University, New Taipei City, Taiwan, ⁴Yuan Ze University, Taoyuan City, Taiwan

The study of neurometabolites and the detection of glucose in the brain has relied on anesthetized animals. Prolonged periods of anesthesia required for these studies may affect these metabolites and the ability to interpret glucose detection in the brain. However, performing post-mortem studies of neurometabolites and glucose detection in the brain is difficult to interpret because of rapid degradation. In this study, we utilized focal beam microwave irradiation (FBMI) without anesthesia to collect brain tissue and determine whether there is postmortem degradation of neurometabolites and glucose using ¹H-NMR and glucoCEST.

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