Hyperpolarized 129Xe gas MRI has been established as an effective tool to investigate lung function in a number of pulmonary diseases. Improved 129Xe polarization techniques provide the opportunity to use a high-resolution 3D static ventilation image acquisition sequence (FGRE) in a 16-second breath-hold. Isotropic-voxel images were acquired in one healthy-volunteer and two patients with asthma; images were compared to coronal images acquired in two other patients with asthma. Isotropic images had sufficient SNR to analyze VDP. This proof-of-concept investigation establishes the feasibility of using an isotropic-voxel acquisition sequence in single breath-hold MRI of the lungs.