Fluorine-19 (19F) magnetic resonance is a powerful tool for tracking fluorine labelled markers, cells and drugs. Here, we studied the influence of temperature on the 19F MR characteristics (chemical shift, T1, T2, SNR) of four fluorinated drugs using 19F MR mapping and spectroscopy techniques. We demonstrated the impact of temperature on the T1 relaxation time of PFCE nanoparticles in vivo and postmortem. Our findings open a trajectory toward 19F MR-based thermometry and indicate the need for adapting MR sequence parameters according to temperature induced environmental changes. This will be an essential requirement for monitoring fluorinated compounds by 19F MR techniques in vivo.