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Abstract #4220

Separation of Intracellular and Extracellular Metabolites during Glucose Fermentation using Diffusion-Weighted Deuterium (2H) Spectroscopy

Josephine B Tan1,2, Rachel W Chan2, Albert P Chen3, and Angus Z Lau1,2

1Medical Biophysics, University of Toronto, Toronto, ON, Canada, 2Physical Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada, 3GE Healthcare, Toronto, ON, Canada

In cancer, high extracellular lactate concentrations are associated with tumour aggressiveness, immune evasion and metastatic potential. We show that diffusion-weighted STEAM 2H-MRS of deuterium-enriched substrates can differentiate between intracellular and extracellular metabolites. Experiments in a model yeast system show the feasibility of detection of intracellular ethanol accumulation, produced via anaerobic fermentation of deuterium enriched glucose. These studies show potential to improve the capability of 2H-MRS for assessment of tumour aggressiveness.

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