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Abstract #4273

Fluorous-metal chelate for sensitive 19F MRI paramagnetic nanoemulsion cell labels

Stephen Adams1, Amin Haghighat-Jahromi2, Chao Wang2, Wenlian Zhu2, Hongyan Xu2, and Eric T Ahrens2

1Department of Pharmacology, UC San Diego, La Jolla, CA, United States, 2Department of Radiology, UC San Diego, La Jolla, CA, United States

Fluorine-19 MRI is a cellular imaging approach enabling quantitative ‘hot-spot’ imaging with no background. The utility of 19F-MRI to detect inflammation and cell therapy in vivo could be expanded by improving the intrinsic sensitivity of the probe by molecular design. We describe a small molecular weight fluorinated metal chelate (SALTAME) based on a salicylidene-tris(aminomethyl)ethane core, with solubility in perfluorocarbon (PFC) oils. SALTAME additive to nanoemulsion is a potent accelerator of 19F T1 and increases the image sensitivity per time with signal averaging. We assessed the biocompatibility, potential 19F signal enhancement and use for detecting inflammation macrophages in mice in vivo.

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