Abstract #4308

Probing multiple metabolic pathways in vivo with 1H-decoupled 13C-MRS of hyperpolarized [2-13C]dihydroxyacetone

Irene Marco-Rius¹, Alan J Wright², De-en Hu², Dragana Savic^3,4, Jack J Miller^3,4,5, Kerstin N Timm³, Damian Tyler^3,4, Kevin M Brindle², and Arnaud Comment^2,6

¹Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain, ²Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, United Kingdom, ³Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom, ⁴Oxford Centre for Clinical Cardiac Magnetic Resonance Research, John Radcliffe Hospital, Headington, United Kingdom, ⁵Department of Physics, University of Oxford, Oxford, United Kingdom, ⁶General Electric Healthcare, Chalfont St Giles, United Kingdom

Hyperpolarized [2-¹³C]dihydroxyacetone is a promising probe to investigate changes in glucose metabolism in different metabolic states, such as the alterations caused by diabetes or non-alcoholic fatty liver disease. Proton decoupled ¹³C-MR spectra of hyperpolarized [2-¹³C]dihydroxyacetone (DHAc) were acquired from the mouse liver using specialized spectral-spatial RF pulses. This optimized protocol for in vivo studies revealed metabolic products from three different metabolic pathways (gluconeogenesis, glycolysis and lipid synthesis) following the injection of this single hyperpolarized substrate. The metabolic products detected include glycerol 3-phosphate, glycerol, phosphoenolpyruvate, lactate, alanine, glyceraldehyde 3-phosphate and glucose 6-phosphate.

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