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Abstract #4322

Non-invasive detection of toxin-induced inflammation in the mouse brain using hyperpolarized 13C MRSI

Lydia Le Page1,2, Caroline Guglielmetti1,2, Brice Tiret1, and Myriam Chaumeil1,2

1Physical Therapy and Rehabilitation Science, University of California, San Francisco, San Francisco, CA, United States, 2Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA, United States

In recent years, hyperpolarized (HP) 13C magnetic resonance spectroscopic imaging (MRSI) has shown promise in assessing neuroinflammation in the brains of mouse models of multiple sclerosis and traumatic brain injury. In this follow-up study, we used 13C MRSI of HP [1-13C] pyruvate/13C urea to assess neuroinflammation following intracranial injection of a toxin, namely lipopolysaccharide (LPS). A significantly elevated lactate:pyruvate ratio was observed in LPS-injected mice, and was associated with a significant increase in the presence of macrophages/microglia and astrocytes as assessed histologically. Our results demonstrate that HP 13C MRS has the sensitivity to assess toxin-induced changes in the brain.

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