Three different MRI methods for obtaining perfusion related parameters were compared and evaluated as biomarkers in a study of 94 rectal cancer patients. The methods were dynamic contrast enhanced (DCE) MRI and dynamic susceptibility contrast (DSC) MRI analysed from a multi-echo dynamic EPI sequence, as well as intravoxel incoherent motion (IVIM) MRI analysed from a diffusion weighted sequence with 7 b-values. Tumour blood flow from DSC MRI was correlated to D* from IVIM MRI as well as Ktrans and vp from DCE MRI. Blood flow was also related to progression free survival, overall survival, treatment response and sex differences.