The association of time-dependent DWI (shifted ADC [sADC], IVIM, and non-Gaussian DWI) at different diffusion times and CEST (MTRasym, APT signal intensity) parameters was investigated with histological biomarkers in a breast cancer xenograft model. ADC values decreased with increased diffusion times. sADC values at a diffusion time=5ms had significant negative correlation with Ki-67 (r=−0.63, P<0.05). MTRasym had a significant positive correlation with Ki-67 positive area (r=0.73, P<0.05). Significant association was found between fIVIM and microvessel density (r=0.80, P<0.01). These results indicate their utility for investigating microstructure and microcirculation of breast cancers without using contrast agents.