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Abstract #0146

Imaging blood-brain barrier disruption caused by CD19 based CAR-T cell immunotherapy

Puneet Bagga1, Stephen Pickup1, Denis Migliorini2, Neil E Wilson1, Mohammad Haris3,4, Suyash Mohan1, Avery D Posey2, and Ravinder Reddy1
1Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, 2Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA, United States, 3Sidra Medicine, Doha, Qatar, 4LARC, Qatar University, Doha, Qatar

With the advent of clinically effective CD19 based chimeric antigen receptor (CAR) T cell immunotherapies, there are incidents of associated neurotoxicity. In this study, we report the use of gadolinium enhanced MRI to image BBB disruption caused by CD1928z CAR-T cell on target action against brain pericytes of immunodeficient non-tumor bearing NSG mice. Pericytes are mural cells that wrap endothelial cells and are critical for maintaining blood-brain-barrier (BBB) integrity and express CD19. The MRI results were also found to corroborate with the Evans blue dye BBB permeability assay.

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