Abstract #0146

Imaging blood-brain barrier disruption caused by CD19 based CAR-T cell immunotherapy

Puneet Bagga¹, Stephen Pickup¹, Denis Migliorini², Neil E Wilson¹, Mohammad Haris^3,4, Suyash Mohan¹, Avery D Posey², and Ravinder Reddy¹

¹Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, ²Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA, United States, ³Sidra Medicine, Doha, Qatar, ⁴LARC, Qatar University, Doha, Qatar

With the advent of clinically effective CD19 based chimeric antigen receptor (CAR) T cell immunotherapies, there are incidents of associated neurotoxicity. In this study, we report the use of gadolinium enhanced MRI to image BBB disruption caused by CD1928z CAR-T cell on target action against brain pericytes of immunodeficient non-tumor bearing NSG mice. Pericytes are mural cells that wrap endothelial cells and are critical for maintaining blood-brain-barrier (BBB) integrity and express CD19. The MRI results were also found to corroborate with the Evans blue dye BBB permeability assay.

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