Parkinson’s Disease (PD) has shown to be associated with cerebrovascular abnormalities, but its non-dopaminergic pathological mechanism is less studied. This study investigated the regulatory effect of levodopa, the most-commonly used therapy for PD, on cerebral arteries and blood flow. 57 PD patients and 17 age-matched healthy controls were scanned for artery morphologic and cerebral perfusion imaging at baseline, then the patients were re-scanned 50 minutes after taking levodopa. Results indicated that levodopa elevated blood perfusion level of PD brains to normal levels and dilated proximal arteries. Plus, blood perfusion showed related to motor syndrome scale post-levodopa.