There is a pressing need for a single-time-point quantitative measure capable of predicting osteoarthritic change. Bilateral knee imaging with T2 cluster asymmetry analysis is a promising approach to achieve this goal. This study examines T2 cluster asymmetry in ACL-injured subjects and controls. ACL-injured subjects showed elevated T2 cluster asymmetry 9-months following reconstruction surgery relative to the controls in the superficial half of cartilage. This novel approach for analyzing T2 relaxation times in femoral cartilage shows promise in detecting changes that may be indicative of early osteoarthritis onset.