Preoperative assessment of histological tumor characteristics plays an essential role in evaluating prognosis and optimizing therapeutic strategies for glioma patients. This study aims to evaluate the feasibility of DKI and APT in differentiating lower-grade glioma (LGG) from glioblastoma at 3T. Twenty-four untreated patients were recruited and classified into LGG (grade II and III, N=10) and glioblastoma (grade VI, N=14). Results show comparable diagnostic performance of APTw and MK in differentiating the two groups with AUCs>0.85, superior to other DKI indices. Combining them further improves the discrimination accuracy, that may greatly facilitate prompt diagnosis and treatment decisions.