Extracellular matrix (ECM) forms the underlying complex structure of bodily tissues, for this reason, ECM has been greatly explored as an ideal scaffold for tissue engineering. To better understand and optimize scaffold-based therapies we require sensitive and non-invasive imaging techniques. In this study, a novel and facile method for labelling and imaging decellularized ECM scaffolds is presented. A series of tissue-specific (bladder, lung, and tracheal smooth muscle and cartilage) dECM scaffolds are labelled with a small molecule manganese porphyrin, MnPNH2. The labelled scaffolds are biocompatible and exhibit significant and sustained signal in vitro and in vivo.