Abstract #0624

An MRI Method for Labeling and Imaging Decellularized Extracellular Matrix Scaffolds for Tissue Engineering

Daniel Andrzej Szulc^1,2, Mohammadali Ahmadipour^1,3, Fabio Gava Aoki^3,4, Thomas K. Waddell^1,3,5, Golnaz of Karoubi^5,6,7, and Hai-Ling Margaret Cheng^1,2,7,8,9

¹Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, ²Translational Biology & Engineering Program, Ted Rogers Centre for Heart Research, Toronto, ON, Canada, ³Latner Thoracic Surgery Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada, ⁴Biomedical Engineering Laboratory, University of Sao Paulo, Sao Paulo, Brazil, ⁵Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, ON, Canada, ⁶Latner Thoracic Surgery Laboratories, University of Toronto, Toronto, ON, Canada, ⁷Ontario Institute for Regenerative Medicine, Toronto, ON, Canada, ⁸Heart & Stroke/Richard Lewar Centre of Excellence for Cardiovascular Research, Toronto, ON, Canada, ⁹Edward S. Rogers Sr. Department of Electrical & Computer Engineering, University of Toronto, Toronto, ON, Canada

Extracellular matrix (ECM) forms the underlying complex structure of bodily tissues, for this reason, ECM has been greatly explored as an ideal scaffold for tissue engineering. To better understand and optimize scaffold-based therapies we require sensitive and non-invasive imaging techniques. In this study, a novel and facile method for labelling and imaging decellularized ECM scaffolds is presented. A series of tissue-specific (bladder, lung, and tracheal smooth muscle and cartilage) dECM scaffolds are labelled with a small molecule manganese porphyrin, MnPNH₂. The labelled scaffolds are biocompatible and exhibit significant and sustained signal in vitro and in vivo.

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