Abstract #0700

Investigation of Dormant and Metastatic Breast Cancer Metabolism via Hyperpolarized 13C-MRS and Fluorescence Lifetime Imaging Microscopy

Paul Begovatz¹, Sarah Erickson-Bhatt^2,3,4, Benjamin Cox², Suzanne Ponik⁴, Kevin Eliceiri^1,2,3, and Sean Fain^1,5,6

¹Medical Physics, University of Wisconsin-Madison, Madison, WI, United States, ²Morgridge Institute for Research, Madison, WI, United States, ³Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, Madison, WI, United States, ⁴Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, United States, ⁵Radiology, University of Wisconsin-Madison, Madison, WI, United States, ⁶Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States

Hyperpolarized ¹³C-Magnetic resonance spectroscopy (¹³C-MRS) and NADH fluorescence lifetime imaging (FLIM) have evolved as methods to detect metabolic shifts in aerobic glycolysis and oxidative phosphorylation which are associated with metastatic potential in cancer metabolism. This study set out to investigate the differences in cancer metabolism between murine non-metastatic, metastatic-dormant, and highly metastatic breast cancer cell lines. FLIM analysis revealed no differences in free and bound NADH between cell lines, indicative of uniform ATP production through oxidative phosphorylation; however, hyperpolarized ¹³C-MRS measurements detected an increase in lactate production, or aerobic glycolysis, which was associated with greater breast cancer metastatic potential.

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