Resolving the different sources of diffusional kurtosis can increase DKI’s specificity towards different microstructural features. Such sources can be resolved using the correlation tensor imaging (CTI) – a novel double diffusion encoding technique that does not rely on common assumptions of time-independent diffusion. Here, a minimal acquisition protocol for CTI is designed and used to characterize the diffusional kurtosis of living rat brains for the first time. We here develop an approach to acquire CTI data in vivo and show that it can robustly decouple inter-compartmental kurtosis sources (anisotropic and isotropic diffusivity variances) from intra-compartmental kurtosis sources.
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