The validation of white matter (WM) tissue modeling for diffusion MRI is challenging, in part, because some of the predicted microstructural parameters (e.g., compartment-specific diffusivities) cannot be easily measured with independent methods such as histology. Most WM tissue models are designed to utilize single diffusion encoding (SDE) MRI data as provided by conventional diffusion MRI sequences. Since multiple diffusion encoding (MDE) MRI yields more information than SDE, it allows for tissue modeling that requires fewer assumptions. Hence, MDE can be applied to help validate the predictions for all SDE model parameters. Here we give an explicit example of this.
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