Abstract #0844

Unprecedented diffusion weighting and exchange resolution of cellular and sub-cellular structures in live and fixed neural tissue

Nathan Hu Williamson¹, Rea Ravin¹, Dan Benjamini¹, Hellmut Merkle², Melanie Falgairolle², Michael J O'Donovan², Dvir Blivis², Dave Ide², Teddy Cai¹, Nima Ghorashi³, Ruiliang Bai^1,4, and Peter Basser¹

¹Eunice Kennedy Shriver National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States, ²National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States, ³National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, United States, ⁴College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China

Diffusion and exchange methods are developed using the large static gradient produced by a single-sided permanent magnet and provide resolution to water within sub-micron membrane structures. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted within membrane structures at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between membrane structures and free environments at 100 s⁻¹.

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