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Abstract #0844

Unprecedented diffusion weighting and exchange resolution of cellular and sub-cellular structures in live and fixed neural tissue

Nathan Hu Williamson1, Rea Ravin1, Dan Benjamini1, Hellmut Merkle2, Melanie Falgairolle2, Michael J O'Donovan2, Dvir Blivis2, Dave Ide2, Teddy Cai1, Nima Ghorashi3, Ruiliang Bai1,4, and Peter Basser1
1Eunice Kennedy Shriver National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States, 2National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States, 3National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, United States, 4College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China

Diffusion and exchange methods are developed using the large static gradient produced by a single-sided permanent magnet and provide resolution to water within sub-micron membrane structures. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted within membrane structures at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between membrane structures and free environments at 100 s−1.

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