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Abstract #0854

Synchrotron microCT tractography connectomics: comparison with diffusion MRI and neural tracer injections

Scott Trinkle1, Sean Foxley1, Narayanan Kasthuri2, and Patrick La Rivière1
1Department of Radiology, The University of Chicago, Chicago, IL, United States, 2Department of Neurobiology, The University of Chicago, Chicago, IL, United States

In this study, we generated tractography-derived, mesoscale mouse “connectomes” from diffusion MRI and synchrotron microCT data on the same mouse brain and evaluated their accuracy using anterograde tracer data from the Allen Mouse Brain Connectivity Atlas. We are developing whole-brain synchrotron microCT, which provides micron-level isotropic resolution across whole mouse brains with no physical sectioning. Even when binning the microCT orientation information to the MRI resolution, microCT tractography outperformed MRI. However, both datasets performed similarly to a tractography connectome constructed entirely with random orientation data, highlighting the importance of understanding geometric biases in tractography connectome construction.

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