In this work, we used our previously reported high-resolution dMRI-based mouse brain atlas8 to trace node-to-node thalamo-cortical structural connectivity in the mouse brain. Taking advantage of the rich viral tracer data from the Allen Mouse Brain Connectivity Atlas (AMBCA)4, the tractography results were examined using the tracer data as ground truth. Our findings pinpoint the potentiality of mapping reliable structural connectivity in gray matter structures using tractography and at the same time, highlight the necessity of further investigation on determining the imaging and tractography parameters for accurate estimation of such connectivity.
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