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Abstract #1195

Tracer kinetic modeling of nitroxide-enhanced MRI to quantify oxidative stress in mouse models of heart disease

Soham Shah1, Yu Wang1, Christopher Waters1, Lanlin Chen1, Brent French1, and Frederick Epstein1
1University of Virginia, Charlottesville, VA, United States

Oxidative stress plays a significant role in the pathogenesis of heart disease. Nitroxide free radicals have been used as redox-sensitive MRI contrast agents where oxidative stress is correlated to the nitroxide-enhanced signal decay rate. We developed a two-compartment exchange and reduction model (2CXRM) to quantify both myocardial nitroxide exchange and reduction and hypothesized that dynamic nitroxide-enhanced MRI can comprehensively assess nitroxide kinetics in mouse models of angiotensin II infusion (ANGII) and myocardial infarction (MI). The 2CXRM detected elevated reduction rates in ANGII and post-MI mice indicative of oxidative stress and reduced nitroxide delivery, consistent with microvascular damage, in post-MI mice.

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