Motivated by recent functional line-scanning recordings in rodents, we developed a procedure to record human cortical layers at high spatial (200 μm) and temporal resolution (100 ms). Our technique addresses challenges associated with human line-scanning, such as planning around cortical folding and restrictive SAR limitations. Our results show that line-scanning of human cortical layers corroborates electrophysiological measurements of tuning properties in primary visual cortex. These results demonstrate that line-scanning is a promising technique for investigating local functional circuits in human cortex.
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