Abstract #1246

Laminar fMRI using layer-specific optogenetic stimulations

Russell W Chan¹, Mazen Asaad², Bradley J Edelman¹, Hyun Joo Lee¹, Hillel Adesnik³, David Feinberg³, and Jin Hyung Lee^1,4,5,6

¹Neurology and Neurological Sciences, Stanford University, Stanford, CA, United States, ²Molecular and Cellular Physiology, Stanford University, Stanford, CA, United States, ³Helen Wills Neuroscience Institute, University of California, Berkeley, CA, United States, ⁴Bioengineering, Stanford University, Stanford, CA, United States, ⁵Neurosurgery, Stanford University, Stanford, CA, United States, ⁶Electrical Engineering, Stanford University, Stanford, CA, United States

We attempted to establish the mesoscale layer-specific fMRI representation of neuronal activity using layer-specific Cre-driver mouse lines, optogenetic stimulations, fMRI and electrophysiological recordings. Although laminar fMRI responses were distinct during L2/3, L4, L5 and L6 stimulations, all fMRI responses increased along the cortical depth. This phenomenon was, however, not observed in LFP and spike recordings. This discrepancy between fMRI, LFP and spiking may be due to the draining veins transporting deoxyhemoglobin from the deeper layers to the superficial layers. Future studies may take into account of neurovasculature to elucidate the exact mechanisms of mesoscale layer-specific neurovascular coupling.

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