Sports-related traumatic brain injuries are difficult to diagnose and prognose due to a lack of standardized metrics. Furthermore, there has been limited research on the effects of repeated sub-concussive and sub-clinical injuries over time. In this study, we characterized changes in resting state connectivity and brain metabolites over a season of collegiate basketball in athletes without a diagnosed concussion. We observed significant changes in resting state connectivity and brain metabolites as a function of game time played. No changes in plasma inflammatory markers were observed over time, suggesting that brain changes were not driven by systemic inflammation.