Radiological diagnosis of certain brain tumours remains poor. The development of automated tools that can extract valuable, yet currently underused, information from routine MRI acquisitions have been explored. The present study aimed to evaluate the accuracy of three automatic tumour segmentation programmes using the BRATS validation 2018 glioma dataset (n=66). The performance of BraTumIA was assessed across an adult glioma dataset from Imperial College Healthcare NHS Trust (n=13) and a paediatric brain tumour dataset from Great Ormond Street Hospital NHS Foundation Trust. DeepMedic segments adult gliomas more accurately than BraTumIA and ONCOhabitats. However, BraTumIA provides a more user-friendly segmentation tool.