To achieve detailed characterization of brain tumors, we demonstrated CEST imaging with multi-pool model [bulk water, magnetization transfer (MT), amide proton transfer (APT), and nuclear Overhauser effect (NOE)] derive from the Bloch equation. This study was performed using five patients with brain tumors. As a result, there was high linearity between MTRasym and T2/T1 in each tumor (R2 = 0.91, P<0.05). Moreover, there were significantly different NOE signals for tumors and normal white matter (P<0.05). Detection using APT and NOE make it possible to provide more detailed information of brain tumors than MTRasym analysis.