In the field of neuroscience, an emerging technology named relaxation quantitative MRI (RQ-MRI) have demonstrated its great potential in both clinic and research. Among those RQ-MRI related technologies, the synthetic MRI has moved rapidly towards clinical application due to its acceptable acquisition time. However, how the spatial resolution of synthetic MRI impacts the quantitative maps is still largely unclear. To address this question, in current study, a total of 13 normal subjects with four distinct spatial resolutions were applied. Our results demonstrated that both the in-plane resolution and slice thickness have significantly influence on the measured quantitative values.