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Abstract #1847

A Container and Detailed Preparation Protocol for Ex Vivo Whole Human Brain MRI

Alan C Seifert1,2,3, Joseph A Borrello1,2,3, Jessie Laffey4, Etty Cortes4, Tamjeed Sikder4, Enna Selmanovic5, Bradley N Delman2, John Crary4, Kristen Dams-O'Connor5, and Junqian Xu1,2,3,6
1Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States, 2Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States, 3Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, United States, 4Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY, United States, 5Department of Rehabilitation Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States, 6Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY, United States

Whole-brain ex vivo MRI has proven extremely valuable in neuroscience research, but detailed descriptions of the specific preparation and packaging steps to minimize motion, susceptibility artifacts, and background signal are often missing from reports of experimental findings. We present the design of a reproducible container and a detailed protocol that yield whole-brain images uncorrupted by susceptibility artifacts. The most important steps to eliminate susceptibility artifacts due to residual air bubbles are removal of the leptomeninges, application of vacuum to dislodge bubbles, immersion in Fluorinert, and rolling of the sealed container, although removal of leptomeninges may be undesirable in some cases.

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