The glymphatic system is a brain waste clearance pathway based on cerebrospinal fluid (CSF) and interstitial fluid exchange. As such, this pathway plays important roles in both acute and neurodegenerative conditions. With 3D-FISP-based fast imaging, we investigated how anesthetic regiments, i.e., ketamine-xylazine (K/X) versus isoflurane, influences the movement of tracer through the brain. This was achieved by conducting acute cisterna magna cannulations, tracer scanning using 3D-FISP, and calculating dynamic tracer distribution maps. Our analysis demonstrates that anesthesia alters the spatial and temporal distribution of CSF tracer, with K/X showing higher influx, and isoflurane shunting CSF out via deep cervical nodes.