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Abstract #1848

High temporal resolution glymphatic influx/efflux analysis with 3D-FISP sequence

Evan Hunter Stanton1, Niklas Daniel Åke Persson1, Björn Sigurdsson1, Tuomas Lilius1, Humberto Mestre2, Maiken Nedergaard1, and Yuki Mori1,3
1Division of Glial Disease and Therapeutics, University of Copenhagen, Center for Translational Neuromedicine, Copenhagen, Denmark, 2Division of Glial Disease and Therapeutics, University of Rochester Medical Center, Center for Translational Neuromedicine, Rochester, NY, United States, 3Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark, Panum NMR Core Facility, Copenhagen, Denmark

The glymphatic system is a brain waste clearance pathway based on cerebrospinal fluid (CSF) and interstitial fluid exchange. As such, this pathway plays important roles in both acute and neurodegenerative conditions. With 3D-FISP-based fast imaging, we investigated how anesthetic regiments, i.e., ketamine-xylazine (K/X) versus isoflurane, influences the movement of tracer through the brain. This was achieved by conducting acute cisterna magna cannulations, tracer scanning using 3D-FISP, and calculating dynamic tracer distribution maps. Our analysis demonstrates that anesthesia alters the spatial and temporal distribution of CSF tracer, with K/X showing higher influx, and isoflurane shunting CSF out via deep cervical nodes.

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