To assess the diagnostic value of CMR Tissue-Tracking(TT) for strain analysis in a rat model of acute myocarditis and the strain’s association with myocardial impairment. Experimental autoimmune myocarditis(EAM) was induced in 16 male rats, 10 rats served as control. Rats were scanned at 7T MRI 21 days after model induction, using cine-FLASH sequence and late gadolinium enhancement imaging. Myocardial strain values significantly reduced in EAM rats, compared with the controls. The area under the curve(AUC) of myocardial strain was excellent. Quantitative analysis of late gadolinium enhancement(LGE) showed a significant correlation with myocardial strain.