Respiratory gating or navigator technique is used in 4D flow imaging to reduce the effect of respiratory motion. Non-respiratory gated 4D flow MRI reduces the overall scan time but may face uncertainty in reproducibility and reliability. In this work, the reliability and reproducibility of non-respiratory gated 4D flow was investigated in 10 patients and 3 different sites in two separate scans. Better measurement consistency was observed intra-site than inter-site. The intra-correlation coefficient results showed excellent to good reproducibility between sites were observed ( ICC > 0.95 for blood flow, ICC > 0.85 for peak). Bland–Altman analysis also showed great test-retest reliability.