Rectal cancer is the third most common cancer and the second cause of cancer-related death worldwide. DWI is now recommended as a routine addition to the MRI protocol for the assessment of rectal cancer. However, the effect of the b-value on the diagnostic performance has not yet been explored. The current study aims to evaluate the value of texture analysis based on DWI with different B values to evaluate the heterogeneity of rectal cancer patients. The skewness and kurtosis were all significantly different in DWI with different B values, and they were obviously changed when B reached 800.