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Abstract #2703

The Molecular Basis for 31P MRS-Based Phosphocreatine Muscle Resynthesis Rate Measurements in Healthy Adults

Maninder Singh1, Moses M Darpolor2, Jeffrey Covington1, Sebastian Hanet1, Eric Ravussin1, and Owen T Carmichael1
1Pennington Biomedical Research Center, Baton Rouge, LA, United States, 2Stillman College, Tuscaloosa, AL, United States

Dynamic phosphorus magnetic resonance spectroscopy (31P-MRS) is a method used for in vivo studies of skeletal muscle function including measurements of phosphocreatine (PCr) synthesis rate during recovery from submaximal exercise. However, the molecular events underlying the PCr resynthesis rate are still under debate. Therefore, our goal was to assess the PCr resynthesis rate from 31P-MRS spectra collected from the skeletal muscle (vastus lateralis) of healthy adults and investigate associations between PCr resynthesis and levels of mitochondria-related transcripts and proteins in the same muscle (NAMPT, NQO1, PGC-1α, and SIRT1).

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