Does freeze/thaw of cadaveric specimens’ damage collagen fibre orientation? Magic angle directional imaging (MADI) in caprine knees assessed the underlying tissue structure before and after freezing for three and six weeks. Tendon thickness reduced after 6 weeks of freezing. Segmented collagen containing voxels decreased by half after freezing. Voids appeared in the internal tendon structure suggestive of ice crystal formation that disrupted collagen fibre orientation. The severity of the structural changes increased the longer the tissue was frozen. Interpreting results with frozen/thawed cadaveric specimens needs care as freezing damages collagen fibre structure that may impact on biomechanical and other properties.