Low back pain (LBP) is a common spinal disease during the middle-to-old-aged population. Degeneration of lumbar intervertebral discs (IVDs) is recognized as the common and crucial cause of LBP. Histogram analysis is a recently popular method for assessing microstructural heterogeneity, however, its diagnostic performance in intervertebral disc degeneration hasn’t been proved. Histogram analysis on axial T2* mapping was applied to characterize degenerative degrees and heterogeneity of discs. All histogram parameters of T2* value were significantly related to Pfirrmann grading. Our study confirmed that the apparent “red zone” on T2* color maps was clearly related to degeneration.