The aim of this study was to apply the atlas-based brain segmentation to high-resolution 3D MRI of the mice brain, to assess a partial volume effect in the voxel. The altas-based segmentation successfully decomposed high-resolution 3D MRI into various anatomical compartments, and by volumetrically analyzing binary masks of the tissue compartment and voxels, a true contribution of the intended tissue compartment in the localized voxel can be assessed. By analyzing the metabolite-specific volume masks, an agreement of the localization between metabolites was evaluated. By evaluating the true contribution of the intended compartment and metabolite-specific agreement, localization reliability can be improved.
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