Separating glutamate (Glu) from its precursor, glutamine (Gln) at 3T is challenging due to signal overlap. Multiple sequence optimisations have been suggested, with simulated data used to assess accuracy. Here, using 7T data as a reference, we investigate the ability of four PRESS sequences with different echo times of separating Glu and Gln. We found that Glu measured at 3T using TE=20ms shows the highest agreement with 7T data. Gln measured using TE=20ms, 30ms and 40ms all agree with Gln measured at 7T. Therefore, we conclude PRESS with TE=20ms is the most effective at separating and quantifying Glu and Gln.