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Abstract #2979

Intracellular sodium changes in cancer cells using a microcavity array-based bioreactor and sodium triple-quantum signal

Dennis Kleimaier1, Victor Schepkin2, Cordula Nies3, Eric Gottwald3, and Lothar R. Schad1
1Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, 2National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, United States, 3Institute of Functional Interfaces, Karlsruhe Institut of Technology, Karlsruhe, Germany

This study demonstrates the possibility of a microcavity array-based bioreactor to investigate intracellular sodium changes using sodium TQ signal. The response of HepG2 cells during 60min Na+/K+-ATPase inhibition by 1mM ouabain or K+-free medium was monitored. An improved TQTPPI sequence with almost four times TQ SNR increase allowed achieving cell sensitivity of 14*106. During cell experiments, the TQ signal increased to 128.9±3.9% and 165.0±7.4% for 1mM ouabain and K+-free medium, respectively. After reperfusion, the TQ signal recovered to 129.5±4.5%. This bioreactor design with improved TQ signal detection provides the capability to investigate a variety of cells using sodium TQ signal.

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