Abstract #2979

Intracellular sodium changes in cancer cells using a microcavity array-based bioreactor and sodium triple-quantum signal

Dennis Kleimaier¹, Victor Schepkin², Cordula Nies³, Eric Gottwald³, and Lothar R. Schad¹

¹Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, ²National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, United States, ³Institute of Functional Interfaces, Karlsruhe Institut of Technology, Karlsruhe, Germany

This study demonstrates the possibility of a microcavity array-based bioreactor to investigate intracellular sodium changes using sodium TQ signal. The response of HepG2 cells during 60min Na⁺/K⁺-ATPase inhibition by 1mM ouabain or K⁺-free medium was monitored. An improved TQTPPI sequence with almost four times TQ SNR increase allowed achieving cell sensitivity of 14*10⁶. During cell experiments, the TQ signal increased to 128.9±3.9% and 165.0±7.4% for 1mM ouabain and K⁺-free medium, respectively. After reperfusion, the TQ signal recovered to 129.5±4.5%. This bioreactor design with improved TQ signal detection provides the capability to investigate a variety of cells using sodium TQ signal.

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