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Abstract #2986

NMR and MRS studies of proteins delivered into cultured cells, towards MR analyses of proteins under physiological conditions

Airi Higashi1, Mitsuhiro Takeda1, Sosuke Yoshinaga1, and Hiroaki Terasawa1
1Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan

We seek to study proteins under physiological conditions, by delivering 13C-labeled proteins into mouse brains and observing them by 13C MRS. We previously observed 13C-labeled a-Synuclein (α-Syn) embedded in an agarose gel by 13C MRS. To examine the feasibility of MRS detection under more in vivo-like conditions, we delivered α-Syn into cultured mammalian cells and observed the cells by NMR, revealing that the delivered α-Syn underwent intracellular interactions and N-terminal acetylation. For MRS experiments with the cells, we constructed a system that perfuses the cells, to prevent cell death over a longer time period.

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