We seek to study proteins
under physiological conditions, by delivering 13C-labeled proteins
into mouse brains and observing them by 13C MRS. We previously
observed 13C-labeled a-Synuclein (α-Syn)
embedded in an agarose gel by 13C MRS. To examine the feasibility of
MRS detection under more in vivo-like
conditions, we delivered α-Syn into cultured mammalian cells and observed the
cells by NMR, revealing that the delivered α-Syn
underwent intracellular interactions and N-terminal acetylation. For MRS
experiments with the cells, we constructed a system that perfuses the cells, to
prevent cell death over a longer time period.
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