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Abstract #2994

Life monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR Spectroscopy

Damian Hertig1,2,3, Sally Maddah1,2, Roman Memedovski1,2, Andrea Debora Felser2, Aitor Moreno4, Matteo Pennestri4, Jean-Marc Nuoffer2,5, and Peter Vermathen1
1Departments of Clinical Research and Radiology, University of Bern, Bern, Switzerland, 2Institute of Clinical Chemistry, University Hospital Bern, Bern, Switzerland, 3Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland, 4Bruker Biospin AG, Fällanden, Switzerland, 5Department of Pediatric Endocrinology, University Hospital Bern, Bern, Switzerland

In this study we show the feasibility of simultaneous measure of oxygen consumption and metabolic data in living 3D cell culture in standard 5mm NMR tube. Using high cell density of 20 million fibroblasts we demonstrate high viability and reproducibility of the measurements over 12 hours. We describe the effect of the flow rate on the metabolic activity. Importantly, we show the required sensitivity to detect substrate degradation rates of major mitochondrial fuel pathways and ability to measure rapid O2 and lactate changes as surrogate marker of oxidative phosphorylation and anaerobic glycolysis.

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