Abstract #2994

Life monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR Spectroscopy

Damian Hertig^1,2,3, Sally Maddah^1,2, Roman Memedovski^1,2, Andrea Debora Felser², Aitor Moreno⁴, Matteo Pennestri⁴, Jean-Marc Nuoffer^2,5, and Peter Vermathen¹

¹Departments of Clinical Research and Radiology, University of Bern, Bern, Switzerland, ²Institute of Clinical Chemistry, University Hospital Bern, Bern, Switzerland, ³Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland, ⁴Bruker Biospin AG, Fällanden, Switzerland, ⁵Department of Pediatric Endocrinology, University Hospital Bern, Bern, Switzerland

In this study we show the feasibility of simultaneous measure of oxygen consumption and metabolic data in living 3D cell culture in standard 5mm NMR tube. Using high cell density of 20 million fibroblasts we demonstrate high viability and reproducibility of the measurements over 12 hours. We describe the effect of the flow rate on the metabolic activity. Importantly, we show the required sensitivity to detect substrate degradation rates of major mitochondrial fuel pathways and ability to measure rapid O₂ and lactate changes as surrogate marker of oxidative phosphorylation and anaerobic glycolysis.

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