Tissue hypoxia induces a variety of detrimental processes. Therefore, the determination of oxygen partial pressure can be helpful to assess the current oxygen supply of an organ. 19F MRI showed to be useful for the quantification of tissue pO2 by exploiting the paramagnetic effect of O2 on the longitudinal relaxation rate R1 of perfluorocarbon nanoemulsions. Thus, allowing the calculation of pO2 through experimental R1 data. In the present study, we applied this approach for monitoring the gradual recovery of tissue oxygenation in a murine model of hind limb ischemia. We observed tissue oxygenation recovery within 2 weeks post-occlusion.