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Abstract #2999

A new approach involving combination of 1H MRS and [2,2,2′-2H3]acetate administration to assess cerebral neurotransmission in vivo

Puneet Bagga1, Laurie J Rich1, Neil E Wilson1, Mark Elliott1, Mitch D Schnall1, John A Detre2, Mohammad Haris3,4, and Ravinder Reddy1
1Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, 2Department of Neurology, University of Pennsylvania, Philadelphia, PA, United States, 3Sidra Medicine, Doha, Qatar, 4LARC, Qatar University, Doha, Qatar

1H MR spectroscopy is currently the only technique that allows the non-invasive detection and quantification of a wide range of neurochemicals. In this study, we performed 1H MRS in conjunction with the administration of [2,2,2′-2H3]acetate to measure turnover kinetics of glutamate, glutamine and GABA in rat brain. As 2H is invisible on 1H MRS, the turnover of metabolites will lead to a corresponding drop in their 1H MR signal visualized by subtraction of the post-[2,2,2′-2H3]acetate administration from the Pre-administration 1H MR spectra. The fractional enrichment data can be fitted to evaluate the rates of cerebral glutamatergic and GABAergic neurotransmitter cycling.

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