Abstract #2999

A new approach involving combination of 1H MRS and [2,2,2′-2H3]acetate administration to assess cerebral neurotransmission in vivo

Puneet Bagga¹, Laurie J Rich¹, Neil E Wilson¹, Mark Elliott¹, Mitch D Schnall¹, John A Detre², Mohammad Haris^3,4, and Ravinder Reddy¹

¹Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, ²Department of Neurology, University of Pennsylvania, Philadelphia, PA, United States, ³Sidra Medicine, Doha, Qatar, ⁴LARC, Qatar University, Doha, Qatar

¹H MR spectroscopy is currently the only technique that allows the non-invasive detection and quantification of a wide range of neurochemicals. In this study, we performed ¹H MRS in conjunction with the administration of [2,2,2′-²H₃]acetate to measure turnover kinetics of glutamate, glutamine and GABA in rat brain. As ²H is invisible on ¹H MRS, the turnover of metabolites will lead to a corresponding drop in their ¹H MR signal visualized by subtraction of the post-[2,2,2′-²H₃]acetate administration from the Pre-administration ¹H MR spectra. The fractional enrichment data can be fitted to evaluate the rates of cerebral glutamatergic and GABAergic neurotransmitter cycling.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.