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Abstract #3015

Real-time Monitoring of in vivo free radical scavengers through hyperpolarized [1-13C] N-acetyl cysteine

Kazutoshi Yamamoto1, Ana Opina2, Keita Saito3, Ronja M Malinowski4, Tomohiro Seki1, Nobu Oshima1, Deepak Sail2, Jeffrey R Brender3, Shun Kishimoto3, Nallathamby Devasahayam1, Jan H Ardenkjær-Larsen4, James B Mitchell5, Rolf E Swenson2, and Murali C Krishna1
1National Cancer Institute, National Institutes of Health, Bethesda, MD, United States, 2Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville, MD, United States, 3National Institutes of Health, Bethesda, MD, United States, 4Department of Electrical Engineering, Technical University of Denmark, Lyngby, Denmark, 5NCI/RBB, National Institutes of Health, Bethesda, MD, United States

N-acetyl cysteine (NAC) is a widely used therapeutic and involved to stimulate glutathione synthesis. Glutathione elevates detoxification and works directly as a free radical scavenger. Here, we synthesized and demonstrated [1-13C]NAC as a promising novel probe for hyperpolarized 13C MRI methodologies, which has limitations in available number of probes. In vivo hyperpolarized NAC was broadly distributed throughout the body. The chemical conversions into products were observed in pancreatic tumor xenografts, Hs766t, and SU.86.86, with various conversion efficiencies depending on metabolic characteristics and status. Hyperpolarized NAC can provide insights into redox status, metabolic profile, and enzymatic activities.

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