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Abstract #3068

In Vivo Tracking and Quantification of Murine Natural Killer Cells via Fluorine-19 MRI

Lawrence M Lechuga1, Matthew H Forsberg2, Kai D Ludwig1, Christian M Capitini2,3, and Sean B Fain1,4,5
1Medical Physics, University of Wisconsin, Madison, Madison, WI, United States, 2Pediatrics, University of Wisconsin, Madison, Madison, WI, United States, 3Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Madison, WI, United States, 4Biomedical Engineering, University of Wisconsin, Madison, Madison, WI, United States, 5Radiology, University of Wisconsin, Madison, Madison, WI, United States

Flourine-19 (19F) MRI has demonstrated the ability to monitor immune cell populations in vivo. Murine green fluorescent protein natural killer (NK) cells were labeled with perfluoropolyether (PFPE-red) and injected intratumorally into lymphoma-bearing mice. In this work, we demonstrate the ability to track and quantify NK cells up to 5 days in vivo, via 19F MRI. In vivo quantification indicated that 88% of the initially detected NK cells remained at the tumor site on day 5. Flow cytometry analysis provided validation that the cellular label remains colocalized within a significant portion of the NK cells at day 5.

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