The selective nature of the blood-brain barrier (BBB) is highly regarded when evaluating the permeability of a molecule such as Cucurbituril (CB6). CB6 has previously demonstrated a strong hyperCEST effect, which makes it a valid candidate for detecting low concentrations of biomarkers of neurodegenerative pathologies. However, CB6 must be permeable to the BBB to use the hyperCEST technique for neurological biomarkers. Therefore, this work evaluates the permeability of CB6 across an artificial BBB based on diffusion. It was found that CB6 can cross the brain sphingomyelin powder (BSM) based artificial BBB.