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Abstract #3170

A method to remove the influence of fixative concentration on post-mortem T2 maps using a kinetic-tensor model

Feng Qi1, Karla Miller1, Sean Foxley2, Menuka Pallebage-Gamarallage3, Ricarda AL Menke1, Olaf Ansorge3, Samuel A Hurley4, and Benjamin C Tendler1
1Wellcome Centre for Integrative Neuroimaging, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom, 2Department of Radiology, University of Chicago, Chicago, IL, United States, 3Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom, 4School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States

Postmortem imaging allows for validation of the origins of image contrast through comparisons with histology. However, the inclusion of formalin fixative substantially reduces the T2. This reduction is (approximately) linear with concentration. Prior to scanning, samples are often placed in a fluid that has more favourable properties for imaging (e.g. perfluorocarbon fluids). This may lead to an outflow of fixative and an increase in T2 at the tissue surface. Here we propose to correct for T2 inhomogeneity by modelling the outflow of fixative within whole, human brains using a novel kinetic tensor model, which incorporates the effects of diffusion anisotropy.

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