We developed a drift-corrected steady pulsed imaging and labeling (dSPIL) to measure CBF with high-resolution and whole brain coverage. We applied a metric of low band pass to remove MRI signal drift using interleaved label-control images. We also optimized several key imaging parameters in an effort to minimize scan time while covering the whole brain. With the optimized dSPIL scheme, quantification of high-resolution CBF in 22 mouse brain regions can be obtained. As a demonstration and validation of the method, the volume of ischemic lesion regions in mice was quantified by calculating CBF reductions.