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Abstract #3952

Network scale optogenetic fMRI with tapered optical fibers

Elizabeth de Guzman1, Alberto Galbusera1, Filippo Pisano2, Elisa Bellistri2, Alexia Stuefer1, Dania Vecchia3, Massimo De Vittorio2,4, Ferruccio Pisanello2, Tommaso Fellin3, and Alessandro Gozzi1
1Functional Neuroimaging Laboratory, Istituto Italiano di Tecnologia, Rovereto, Italy, 2Center for Biomolecular Nanotechnologies, Istituto Italiano di Tecnologia, Arnesano, Italy, 3Optical approaches to brain function, Istituto Italiano di Tecnologia, Genova, Italy, 4Dip. di Ingegneria dell’Innovazione, Università del Salento, Lecce, Italy

Optogenetic fMRI is an attractive tool for studying neuronal manipulation dynamics at multiple scales, but currently lacks techniques for network (i.e. mesoscale) level investigations due to the small illumination volume of traditional flat-faced optical fibers. Here we show that a recently developed tapered fiber design allowed simultaneous, artefact-free illumination of large cortico-hippocampal areas. We also demonstrated that low-frequency cortico-hippocampal entrainment resulted in a prominent functional desynchronization of the mouse default mode network. These results support the use of this technique for deconstructing network-scale substrates and probing brain-wide functional network dynamics.

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